results for au:Altenbuchinger_M in:q-bio

- Jan 26 2018 q-bio.QM arXiv:1801.08447v1The gene expression profile of a tissue averages the expression profiles of all cells in this tissue. Digital tissue deconvolution (DTD) addresses the following inverse problem: Given the expression profile $y$ of a tissue, what is the cellular composition $c$ of that tissue? If $X$ is a matrix whose columns are reference profiles of individual cell types, the composition $c$ can be computed by minimizing $\mathcal L(y-Xc)$ for a given loss function $\mathcal L$. Current methods use predefined all-purpose loss functions. They successfully quantify the dominating cells of a tissue, while often falling short in detecting small cell populations. Here we learn the loss function $\mathcal L$ along with the composition $c$. This allows us to adapt to application-specific requirements such as focusing on small cell populations or distinguishing phenotypically similar cell populations. Our method quantifies large cell fractions as accurately as existing methods and significantly improves the detection of small cell populations and the distinction of similar cell types.
- Mar 23 2017 q-bio.QM arXiv:1703.07724v1Motivation: Metabolomics data is typically scaled to a common reference like a constant volume of body fluid, a constant creatinine level, or a constant area under the spectrum. Such normalization of the data, however, may affect the selection of biomarkers and the biological interpretation of results in unforeseen ways. Results: First, we study how the outcome of hypothesis tests for differential metabolite concentration is affected by the choice of scale. Furthermore, we observe this interdependence also for different classification approaches. Second, to overcome this problem and establish a scale-invariant biomarker discovery algorithm, we extend linear zero-sum regression to the logistic regression framework and show in two applications to ${}^1$H NMR-based metabolomics data how this approach overcomes the scaling problem. Availability: Logistic zero-sum regression is available as an R package as well as a high-performance computing implementation that can be downloaded at https://github.com/rehbergT/zeroSum.